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KMID : 0043319820050020045
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Archives of Pharmacal Research
1982 Volume.5 No. 2 p.45 ~ p.52
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Stabilization of beta-D-galactosidase from Heat and Chemical Inactivation with the Extract of Panax ginseng C.A. Meyer
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Kim DH
Hahn YH/Hong SK
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Abstract
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Stablization effect of Panax ginseng C. A. Meyer on beta-D-galactosidase inactivation was proved by kinetic studies of thermal inactivation of the enzyme. The water extract Panax ginseng C.A. Meyer showed stabilization activity at minimal concentration of 10ppm. The methanolic extract was purified to obtain ginseng saponins, and two groups of the ginsenosides, i.e., protopanaxadiol and protopanaxatriol were isolated. They also showed a protective effect against the thermal and chemical inactivation of the enzyme; p-chloromercuribenzoic acid and hydroxylamine known as protein modifier greatly inactivated the enzyme but inactivation was significantly blocked by the ginseng component. Mg2+. known as a cofactor, stabilized the enzyme and the poor stabilization effect by it was potentiated by ginseng components.
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KEYWORD
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